abstract：:In vivo hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient T cells (MT) from melanoma patients are enriched for T cells with in vivo clonal amplifications that traffic between blood and tumor tissues. Melanoma is thus a model cancer to test the hypothesis that in vivo MT from cancer patients can be used a...

journal_title：Journal of immunological methods

authors： Zuleger CL,Macklin MD,Bostwick BL,Pei Q,Newton MA,Albertini MR

更新日期：2011-02-28 00:00:00

abstract：:Staphylococcus aureus causes a variety of infections. Knowledge about the physiological role of most S. aureus antigens in colonization and infection is only limited. This can be studied by measuring antigen-specific antibody responses. In this study, we optimized the multiplex microsphere bead-based flow cytometry te...

journal_title：Journal of immunological methods

authors： van den Berg S,Bowden MG,Bosma T,Buist G,van Dijl JM,van Wamel WJ,de Vogel CP,van Belkum A,Bakker-Woudenberg IA

更新日期：2011-02-28 00:00:00

abstract：:Endosialin/TEM1 is predominantly expressed on neovasculature, thus ideally suited for diagnostic, targeted imaging and therapy of cancer. To isolate TEM1-specific affinity reagents, we thought to screen a recombinant antibody (scFv) library derived from the repertoire of a patient with thrombotic thrombocytopenic purp...

journal_title：Journal of immunological methods

authors： Zhao A,Nunez-Cruz S,Li C,Coukos G,Siegel DL,Scholler N

更新日期：2011-01-05 00:00:00

abstract：:Flow cytometry offers a promising alternative to the current methods of amniocentesis or chorionic villus sampling (CVS) for fetal cell sorting for prenatal diagnosis. While flow cytometric methods have been greatly improved to be more sensitive at detecting fetal cells within the maternal circulation, there are still...

journal_title：Journal of immunological methods

authors： Curtis MG,Walker B,Denny TN

更新日期：2011-01-05 00:00:00

abstract：:Pathologic rheumatoid factor (RF) levels are hallmarks of several human diseases. Production of monoclonal RF in vitro is essential for studies of the antigenic specificities of RF, as well as for a dissection of the mechanisms of aberrant RF+ B cell activation. We have expanded upon previous methods to develop a flow...

journal_title：Journal of immunological methods

authors： Charles ED,Orloff MI,Dustin LB

更新日期：2011-01-05 00:00:00

abstract：:Electrochemiluminescence (ECL) assays have been widely used for the detection of anti-therapeutic antibodies (ATAs) against biotherapeutics. With the discontinuation of BioVeris (BV) ECL platform, an alternative technology was needed to replace BV assays to ensure continuous support of multi-year clinical studies. Aft...

journal_title：Journal of immunological methods

authors： Qiu ZJ,Ying Y,Fox M,Peng K,Lewin-Koh SC,Coleman D,Good J,Lowe J,Rahman A,Yang J,Jiang J,Quarmby V,Song A

更新日期：2010-10-31 00:00:00

abstract：:In this paper we provide both a theoretical and experimental analysis of the sensitivity of a DNA quantitation assay using a fluorescent chromophore which non-covalently binds dsDNA. It is well-known that the range of DNA concentrations available for fluorescence quantitation depends on the concentration of the chromo...

journal_title：Journal of immunological methods

authors： Dragan AI,Bishop ES,Casas-Finet JR,Strouse RJ,Schenerman MA,Geddes CD

更新日期：2010-10-31 00:00:00

abstract：:Use of fluorescently labelled multimers, particularly tetramers of peptide and MHC class I glycoprotein (pMHC-I) complexes, is essential for the analysis of CD8+ T cell immunity in basic research and clinical settings. A recently described combinatorial approach using pMHC-I multimers coupled to a unique combination o...

journal_title：Journal of immunological methods

authors： Cukalac T,Valkenburg SA,La Gruta NL,Turner SJ,Doherty PC,Kedzierska K

更新日期：2010-08-31 00:00:00

abstract：:Transduction of bone marrow stem cells with retroviral expression vectors represents a cheaper and more rapid alternative to conventional transgenesis for studies of in vivo gene function. However, achieving tissue-specific expression of genes inserted into retroviral vectors is notoriously difficult. We have develope...

journal_title：Journal of immunological methods

authors： Turner VM,Gardam S,Brink R

更新日期：2010-08-31 00:00:00

abstract：:Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for the isolation of mouse blood monocytes remains a hurdle. Here we describe a fast and easy method for isolating monocytes from mouse blood based o...

journal_title：Journal of immunological methods

authors： Houthuys E,Movahedi K,De Baetselier P,Van Ginderachter JA,Brouckaert P

更新日期：2010-07-31 00:00:00

abstract：:The aim of this study was to evaluate the influence on the results of the main variables of ELISA used for the detection of antiphosphatidylethanolamine antibodies (aPE). Forty sera from patients with either autoimmune disorders including antiphospholipid syndrome (APS) or the clinical features of APS only were assaye...

journal_title：Journal of immunological methods

authors： Sanmarco M

更新日期：2010-06-30 00:00:00

abstract：:The performance of immunoassays for the detection of autoantibodies is of critical importance to the diagnosis and assessment of patients with systemic lupus erythematosus (SLE). Our objective was to compare 3 multiplexed assays for measurement of multiple autoantibodies and their association with global disease activ...

journal_title：Journal of immunological methods

authors： Hanly JG,Su L,Farewell V,Fritzler MJ

更新日期：2010-06-30 00:00:00

abstract：:2-Methoxyestradiol (2ME), an end-metabolite of 17beta-estradiol, is an antiproliferative agent that is currently being tested in clinical trials for cancer treatment. We hereby report that sub-cytotoxic concentrations of 2ME influence the in vitro proliferation of human peripheral blood B lymphocytes. More surprisingl...

journal_title：Journal of immunological methods

authors： Cayer MP,Drouin M,Proulx M,Jung D

更新日期：2010-04-15 00:00:00

abstract：:NK cells are lymphocytes of the innate immune system that can kill target cells after activation signal-induced directional secretion of lytic granule contents. This process depends upon F-actin polymerization at the NK cell immunological synapse (NKIS), which is the dynamic organization of molecules at the interface ...

journal_title：Journal of immunological methods

authors： Banerjee PP,Orange JS

更新日期：2010-04-15 00:00:00

abstract：:IL-7 is a crucial cytokine for T cell hematopoiesis and peripheral homeostasis which by signaling through its receptor alpha chain (CD127) is essential for inducing T cell proliferation and survival. Since the specific CD127 alpha chain is found in a soluble state (sCD127) and at a high level in plasma (ng/mL), it is ...

journal_title：Journal of immunological methods

authors： Janot-Sardet C,Assouline B,Cheynier R,Morre M,Beq S

更新日期：2010-02-28 00:00:00

abstract：:Human phospholipase B-precursor (PLB-P) is a newly identified and purified protein from human neutrophils. The precise function of PLB-P in vivo is not yet known. Its existence in neutrophils and the enzymatic activity against phospholipids imply a role in the defence against invading microorganisms and in the generat...

journal_title：Journal of immunological methods

authors： Xu S,Cai L,Zhao L,Douhan-Håkansson L,Kristjánsson G,Pauksen K,Venge P

更新日期：2010-02-28 00:00:00

abstract：:Natural CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers an...

journal_title：Journal of immunological methods

authors： Becker C,Hoschützky H,Rist W,Lenter M,Schneider FJ,Jonuleit H

更新日期：2010-02-28 00:00:00

abstract：:Neutrophil accumulation at an inflammatory site or an infected tissue is dependent on the recognition of chemotactic peptides that bind to G-protein coupled receptors (GPCRs) exposed on the surface of the inflammatory cells. A GPCR activated by a chemoattractant quickly becomes refractory to further stimulation by lig...

journal_title：Journal of immunological methods

authors： Karlsson J,Bylund J,Movitz C,Björkman L,Forsman H,Dahlgren C

更新日期：2010-01-31 00:00:00

abstract：:LRP6 is a cell surface molecule that plays a critical role in the Wnt signaling pathway, and is implicated in numerous human diseases. Studies of cellular signaling mediated by LRP6 have relied on overexpression experiments, due to the lack of good monoclonal antibodies (mAbs) reactive with native LRP6 ectodomain. By ...

journal_title：Journal of immunological methods

authors： Yasui N,Mihara E,Nampo M,Tamura-Kawakami K,Unno H,Matsumoto K,Takagi J

更新日期：2010-01-31 00:00:00

abstract：:Differentiating foot-and-mouth disease virus (FMDV) antibodies generated during a natural infection from those due to vaccination (DIVA) is crucial for proving freedom from disease after an outbreak and allowing resumption of trade in livestock products. The World Organisation for Animal Health (OIE) recommends that F...

journal_title：Journal of immunological methods

authors： Muller JD,Wilkins M,Foord AJ,Dolezal O,Yu M,Heine HG,Wang LF

更新日期：2010-01-31 00:00:00

abstract：:The common marmoset is a small New World Primate that has been used as an immunological model for a number of human diseases. Dendritic cells (DC) have not been extensively characterised in this species and in particular protocols to derive DC from living donors without the need for animal sacrifice are presently lack...

journal_title：Journal of immunological methods

authors： Prasad S,Kireta S,Leedham E,Russ GR,Coates PT

更新日期：2010-01-31 00:00:00

abstract：:Wnt5a is a noncanonical member of the Wnt family of signaling molecules that has been linked to various physiological and pathological processes including cell differentiation, cell migration, cell growth, vascular remodeling, cancer and chronic inflammation. To understand the role of Wnt5a in these processes, it is n...

journal_title：Journal of immunological methods

authors： Kummitha CM,Mayle KM,Christman MA 2nd,Deosarkar SP,Schwartz AL,McCall KD,Kohn LD,Malgor R,Goetz DJ

更新日期：2010-01-31 00:00:00

abstract：:AMPKalpha is a subunit of AMP-activated protein kinase (AMPK), a heterotrimeric enzyme that works as a fuel sensor activated in response to the depletion of cellular ATP. AMPKalpha is considered as a master switch in regulating glucose and lipid metabolism. Determining its presence in patient sera may help in diagnosi...

journal_title：Journal of immunological methods

authors： Malvoisin E,Livrozet JM,El Hajji-Ridah I,Makloufi D,Vincent N

更新日期：2009-12-31 00:00:00

abstract：:In order to isolate monoclonal antibodies (mAbs) that bind to tumor-associated antigens (Ags) we developed the following strategy. Using the phage-display Ab library we isolated a large number of mAbs that bind to the surface of human tumor cells. The mAbs were individually screened by immunostaining, and clones that ...

journal_title：Journal of immunological methods

authors： Kurosawa G,Sumitomo M,Akahori Y,Matsuda K,Muramatsu C,Takasaki A,Iba Y,Eguchi K,Tanaka M,Suzuki K,Morita M,Sato N,Sugiura M,Sugioka A,Hayashi N,Kurosawa Y

更新日期：2009-12-31 00:00:00

abstract：:Testing of diphtheria vaccines for routine lot release relies heavily on the use of in vivo potency assays. However, consistency of production is also recognised as an important feature of vaccine manufacture, and in vitro assays are superior to in vivo assays for providing this information. In adsorbed vaccines, anti...

journal_title：Journal of immunological methods

authors： Coombes L,Stickings P,Tierney R,Rigsby P,Sesardic D

更新日期：2009-10-31 00:00:00

abstract：:In this study, we developed a one-step, washing-free and amplification-free assay for protein analysis using gold nanoparticle probes (GNPs) and dynamic light scattering (DLS) technique. The target protein concentration was determined by analyzing the level of GNP aggregation caused by antibody-antigen interactions us...

journal_title：Journal of immunological methods

authors： Liu X,Huo Q

更新日期：2009-09-30 00:00:00

abstract：:To date, studies on T cells in acute myeloid leukemia (AML) have been limited to flow cytometric analysis of whole peripheral blood mononuclear cell (PBMC) specimens or functional work looking at the impact of AML myeloblasts on normal or remission T cells. This lack of information on T cells at the time of presentati...

journal_title：Journal of immunological methods

authors： Le Dieu R,Taussig D,Lister TA,Gribben JG

更新日期：2009-08-31 00:00:00

abstract：:Granulocytes and monocytes/macrophages represent key effector cells of the innate immune system. While human monocytes have been recognized as capable of secreting a broad spectrum of cytokines, the situation has been less clear in granulocytes with studies often showing conflicting results. In this study, lipopolysac...

journal_title：Journal of immunological methods

authors： Smedman C,Gårdlund B,Nihlmark K,Gille-Johnson P,Andersson J,Paulie S

更新日期：2009-07-31 00:00:00

abstract：:Macrophages are an important part of the cellular immune system and play a key role during immune responses. Thus, macrophages are interesting targets in basic and clinical research. Primary monocytes or monocyte-derived macrophages do not proliferate on a suitable scale so that their use for functional studies in vit...

journal_title：Journal of immunological methods

authors： Schnoor M,Buers I,Sietmann A,Brodde MF,Hofnagel O,Robenek H,Lorkowski S

更新日期：2009-05-31 00:00:00

abstract：:Feasibility, amount of sample aliquots, processing time and cost are critical considerations for optimizing and conducting assays for large-population based studies. Well designed statistical approaches that quickly identify optimal conditions for a given assay could assist efficient completion of the laboratory assay...

journal_title：Journal of immunological methods

authors： Ryan JE,Dhiman N,Ovsyannikova IG,Vierkant RA,Pankratz VS,Poland GA

更新日期：2009-02-28 00:00:00

abstract：:Neutrophils are a critical component of the innate immune response to invading microbial pathogens. However, an excessive and/or prolonged neutrophil response can result in tissue injury that is thought to underlie the pathogenesis of various inflammatory diseases. The development of novel therapeutic strategies for i...

journal_title：Journal of immunological methods

authors： Zemans RL,Briones N,Young SK,Malcolm KC,Refaeli Y,Downey GP,Worthen GS

更新日期：2009-01-30 00:00:00

abstract：:Natural Killer cells are cells of the innate immune system that are important for the recognition and clearance of virally infected cells or tumors. Examination of the development and signaling of these cells has been severely hampered due to an inability to over-express proteins in these cells. We developed a novel t...

journal_title：Journal of immunological methods

authors： Orr SJ,Quigley L,McVicar DW

更新日期：2009-01-30 00:00:00

abstract：:An ultra-sensitive quantitative EPO (erythropoietin) lateral flow immunochromatographic test with a detection limit of 1.2 fM (10(-15) M), 0.035 ng EPO/L, which is 50-100 times more sensitive than a corresponding enzyme based immunoassay, is presented. In comparison with commercial lateral flow tests for other analyte...

journal_title：Journal of immunological methods

authors： Lönnberg M,Drevin M,Carlsson J

更新日期：2008-12-31 00:00:00

abstract：:Previously we have described studies on in vitro pentamer assembly of Escherichia coli (E. coli) derived heat-labile enterotoxin B subunit (EtxB) using conventional monoclonal antibodies (Amin et al., JBC 1995: 270, 20143-50 and Chung et al., JBC 2006: 281, 39465-70). To extend these studies further we have used phage...

journal_title：Journal of immunological methods

authors： Chung WY,Sack M,Carter R,Spiegel H,Fischer R,Hirst TR,Williams NA,James RF

更新日期：2008-12-31 00:00:00

abstract：:The T cell receptor (TCR) determines the cellular response to antigens, which are presented on the surface of target cells in the form of a peptide bound to a product of the major histocompatibility complex (pepMHC). The response of the T cell depends on the affinity of the TCR for the pepMHC, yet many TCRs have been ...

journal_title：Journal of immunological methods

authors： Chervin AS,Aggen DH,Raseman JM,Kranz DM

更新日期：2008-12-31 00:00:00

abstract：:Bromodeoxyuridine (BrdU) is used to label synthesizing DNA and to chase label-retaining cell (LRC). As stem cells divide slowly in adult tissues, they can be visualized as LRCs. In order to identify LRCs in hard tissue, we examined optimal conditions of fixation, demineralization, and DNA denaturation/antigen retrieva...

journal_title：Journal of immunological methods

authors： Shimada A,Shibata T,Komatsu K,Nifuji A

更新日期：2008-11-30 00:00:00

abstract：:Although enveloped retroviruses bud from the cell surface of T lymphocytes, they use the endocytic pathway and the internal membrane of multivesicular bodies for their assembly and release from macrophages and dendritic cells (DCs). Exosomes, physiological nanoparticles produced by hematopoietic cells, egress from thi...

journal_title：Journal of immunological methods

authors： Cantin R,Diou J,Bélanger D,Tremblay AM,Gilbert C

更新日期：2008-09-30 00:00:00

abstract：:Hereditary angioedema (HAE) is an autosomal dominant disease characterized by recurrent episodes of potentially life-threatening angioedema. The most widespread underlying genetic deficiency is a heterozygous deficiency of the serine protease inhibitor C1 esterase inhibitor (C1-Inh). In addition to low C4 levels, the ...

journal_title：Journal of immunological methods

authors： Wagenaar-Bos IG,Drouet C,Aygören-Pursun E,Bork K,Bucher C,Bygum A,Farkas H,Fust G,Gregorek H,Hack CE,Hickey A,Joller-Jemelka HI,Kapusta M,Kreuz W,Longhurst H,Lopez-Trascasa M,Madalinski K,Naskalski J,Nieuwenhuys E,P

更新日期：2008-09-30 00:00:00

abstract：:Single chain antibody fragment genes are commonly created by splicing together the immunoglobulin light chain (VL) and heavy chain variable (VH) genes of a monoclonal antibody produced by a hybridoma. Selective PCR amplification of the functional immunoglobulin variable gene rearrangements can be complicated by the ex...

journal_title：Journal of immunological methods

authors： Irani Y,Tea M,Tilton RG,Coster DJ,Williams KA,Brereton HM

更新日期：2008-07-31 00:00:00

abstract：:We sought to develop and optimize a hybridoma-based technology for generating human hybridomas that secrete virus-specific monoclonal antibodies for clinical diagnosis and therapy. We developed a novel electrofusion protocol for efficiently fusing Epstein-Barr virus (EBV)-transformed human B cells with myeloma partner...

journal_title：Journal of immunological methods

authors： Yu X,McGraw PA,House FS,Crowe JE Jr

更新日期：2008-07-31 00:00:00